Scaling up iPS cells

Grown up mice from gene-corrected iPS cells.

In 2008, Science named induced pluripotent stem cells (iPS) the breakthrough of the year.1 In 2009, Nature Methods called the process of generating iPS cells the “Method of the Year.“2 Two years later, although the use of iPS cells remains a bright promise for both research and regenerative medicine, concerns remain about how the epigenetic and genetic changes that occur in the creation of iPS ...

Aging in iPS cells

(iPSC) technology [1] holds the promise of recreating disease in vitro in a patient-specific manner. There has been considerable success in using iPSC models to study early-onset genetic diseases, such as our own work on familial dysautonomia [2] or primary herpes simplex encephalitis [3]. The modeling of late-onset diseases in iPSC-derived lineages has been more challenging and such studies of...

Scaling Up

Scaling Up

Most analytical and experimental results on evolutionary programming (EP) are obtained using low-dimensional problems, e.g., smaller than 50. It is unclear, however, whether the empirical results obtained from the low-dimensional problems still hold for high-dimensional cases. This paper investigates the behaviour of four diierent EP algorithms for large-scale problems, i.e., problems whose dim...

iPs cells without antibiotic selection

nature methods | VOL.11 NO.3 | MARCH 2014 | 291 nucleases should be replaced with nickases to avoid off-target mutations13,14. However, using nickases to improve fidelity also lowers efficiency, which makes mutants increasingly rare13,14. Therefore, genome engineering is faced with a logistical challenge: high-fidelity precise mutagenesis results in rarer mutagenic events, but isolating a rare ...